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Pyridostigmine

Prescription
Acetylcholinesterase Inhibitor / Anticholinesterase
Last reviewed 21 Apr 2026 · PetCare.AI Editorial Team
Species
Dog, Cat
Brands
2 available
Formulations
4

Mechanism of action

Reversibly inhibits acetylcholinesterase at the neuromuscular junction, allowing acetylcholine to accumulate and bind nicotinic receptors on skeletal muscle, improving neuromuscular transmission. Used for myasthenia gravis management.

At a glance

Class
Acetylcholinesterase Inhibitor / Anticholinesterase
Schedule
Prescription
Storage
Store at room temperature below 25°C, protect from moisture; injectable refrigerated

Dosing

🐕

Dog

Treatment of choice for generalized and focal myasthenia gravis in dogs
Dose
0.5–3 mg/kg
Route
PO
Frequency
q8–12h (IR) or q12h (SR)
🐈

Cat

Feline myasthenia gravis is less common but documented
Dose
0.25 mg/kg
Route
PO
Frequency
q8–12h
Educational reference only
This information is provided for educational purposes and is not a substitute for professional veterinary advice, diagnosis, or treatment. Always consult a qualified veterinarian before administering any medication to your pet. Find a vet near you →

Formulations

💊

Other — 4

Strength
Strength
Strength
Strength

Storage

Store at room temperature below 25°C, protect from moisture; injectable refrigerated

Safety

Monitoring parameters

Muscle strength (response to Tensilon test if in crisis)Signs of cholinergic toxicity vs. myasthenic weaknessChest radiograph (aspiration pneumonia risk)
Educational reference only
This information is provided for educational purposes and is not a substitute for professional veterinary advice, diagnosis, or treatment. Always consult a qualified veterinarian before administering any medication to your pet. Find a vet near you →

Interactions

No documented interactions.

Brands

Other markets

Mestinon
Regonol

FAQs

Frequently asked questions

What is Pyridostigmine?
Pyridostigmine is a acetylcholinesterase inhibitor / anticholinesterase used in pets. Reversibly inhibits acetylcholinesterase at the neuromuscular junction, allowing acetylcholine to accumulate and bind nicotinic receptors on skeletal muscle, improving neuromuscular transmission. Used for myasthenia gravis management.
What is Pyridostigmine used for in pets?
Pyridostigmine is used in veterinary medicine for: Treatment of choice for generalized and focal myasthenia gravis in dogs; Feline myasthenia gravis is less common but documented.
What is the Pyridostigmine dose for dogs?
For dogs, Pyridostigmine is typically dosed as follows — Treatment of choice for generalized and focal myasthenia gravis in dogs: 0.5–3 mg/kg PO q8–12h (IR) or q12h (SR). Always consult your veterinarian for a dose tailored to your pet's weight, age, and condition.
What is the Pyridostigmine dose for cats?
For cats, Pyridostigmine is typically dosed as follows — Feline myasthenia gravis is less common but documented: 0.25 mg/kg PO q8–12h. Always consult your veterinarian for a dose tailored to your pet's weight, age, and condition.
Does Pyridostigmine need a prescription?
Yes. Pyridostigmine is a prescription medication and should only be administered under veterinary supervision.
Educational reference only
This information is provided for educational purposes and is not a substitute for professional veterinary advice, diagnosis, or treatment. Always consult a qualified veterinarian before administering any medication to your pet. Find a vet near you →

References

References

The PetCare.AI drug reference is built from 13 authoritative sources cited across 580 drug monographs.

Textbooks & handbooks — 5

  • Plumb's Veterinary Drug Handbook
  • Withrow & MacEwen's Small Animal Clinical Oncology
  • Merck Veterinary Manual
  • NRC Nutrient Requirements of Dogs and Cats
  • Veterinary Pharmacology and Therapeutics (Riviere & Papich)

Clinical guidelines & consensus — 4

  • ASPCA Animal Poison Control Center Guidelines
  • AAHA Diabetes Management Guidelines
  • ASPCA Poison Control Guidelines
  • RECOVER CPR Guidelines

Journals & peer-reviewed studies — 2

  • EPIC Study (J Vet Intern Med 2016)
  • JVIM FAT CAT Study

Regulatory & approvals — 1

  • CDSCO Veterinary Drug Approval Registry (1969–2026)

Databases — 1

  • Washington State University VCPL MDR1 Database
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